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TEF-3 Antibody (B-5): sc-390578

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Datasheets
  • TEF-3 Antibody (B-5) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 141-178 mapping within an internal region of TEF-3 of human origin
  • Anti-TEF-3 Antibody (B-5) is recommended for detection of TEF-3 of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-TEF-3 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • TransCruz reagent for ChIP application (sc-390578 X, 200 µg/0.1 ml)
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TEF-3 (B-5): sc-390578.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TEF-3 Antibody (B-5) for WB applications. These reagents are now offered in bundles with TEF-3 Antibody (B-5) (see ordering information below).

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TEF-3 Antibody (B-5) is an IgG1 κ mouse monoclonal TEF-3 antibody that detects TEF-3 of mouse, rat and human origin by WB, IP, IF and ELISA. TEF-3 Antibody (B-5) is available as the non-conjugated anti-TEF-3 antibody form, as well as multiple conjugated forms of anti-TEF-3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. TEF-3, also known as TEAD4 (TEA domain family member 4), RTEF1, EFTR-2, TEFR-1, TCF13L1 or hRTEF-1B, is a 427 amino acid member of the transcriptional enhancer factor (TEF) family of proteins that are characterized by the presence of a TEA DNA-binding domain. Localized to the nucleus and expressed primarily in skeletal muscle, TEF-3 functions as a transcriptional regulator by binding specifically and non-cooperatively to the M-CAT motif found in the promotors of muscle-specific genes, thereby directing their subsequent expression. TEF-3 contains one TEA DNA-binding domain and is expressed as multiple isoforms due to alternative splicing events.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Is any data available on this antibody efficiency for IF?

Asked by: RRoya
This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates! I would recommend an alternative, TEF-3 Antibody (N-G2): sc-101184, which has numerous product citations using the antibody for IF and IHC applications.
Answered by: Technical Support
Date published: 2020-04-22

For Western Blot, is it recommended to use denatured or non-denatured conditions with TEF-3 (B-5): sc-390578 monoclonal antibody?

Asked by: Cweed
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from It works fine for western blottingI used this ab sample a few days ago. It works fine for western blotting
Date published: 2021-11-10
Rated 5 out of 5 by from TEF-3 AntibodyTHIS ANTIBODY IS VERY GOOD, VERY GOOD.VTHE BAND SIZE IS CORRECT
Date published: 2018-04-10
Rated 5 out of 5 by from Good Western blot data of TEFGood Western blot data of TEF-3 expression in non-transfected and human TEF-3 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-06-17
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TEF-3 Antibody (B-5) is rated 4.7 out of 5 by 3.
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