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IL-6Rα Antibody (H-7): sc-373708
- IL-6Rα Antibody (H-7) is a mouse monoclonal IgG1 κ, cited in 26 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 441-468 at the C-terminus of IL-6Rα of human origin
- Anti-IL-6R alpha Antibody (H-7) is recommended for detection of IL-6Rα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-IL-6R alpha Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IL-6Rα (H-7): sc-373708.
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for IL-6Rα Antibody (H-7) for WB and IHC(P) applications. This reagent is now offered in a bundle with IL-6Rα Antibody (H-7) (see ordering information below).
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IL-6Rα Antibody (H-7) is an IgG1 κ mouse monoclonal IL-6Rα antibody (also designated IL-6Rα antibody or Interleukin 6 Receptor antibody) that detects IL-6Rα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. IL-6Rα Antibody (H-7) is available as the non-conjugated anti-IL-6Rα antibody form, as well as multiple conjugated forms of anti-IL-6Rα antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. IL-6 activates intracellular signaling through binding a receptor consisting of a ligand-binding protein (IL-6Rα) and a second protein. IL-6 first binds to IL-6Rα (also known as gp80), which subsequently associates with a gp130 dimer. The active signaling complex consists of, at minimum, IL-6, IL-6Rα and a dimer of two gp130 proteins that are linked by a disulfide bond. A soluble form of IL-6Rα, namely sIL-6Rα, is generated by proteolytic cleavage of the membrane-bound precursor and can function as an agonistic molecule that can actively participate in cell-to-cell signaling. The second subunit of the IL-6 complex, gp130, also functions as a component of several additional receptor complexes, including leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and IL-11. LIF binds to the LIF receptor with low affinity and to a complex of the LIF receptor and gp130 with high affinity, while OSM appears to bind to gp130 with low affinity and to a complex of gp130 and the LIF receptor with high affinity.
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