CUTL1/CUX1/CDP Antibody (B-10): sc-514008

CDP Antibody (B-10) is an IgG₃ κ mouse monoclonal CDP antibody (also designated CUX1 antibody, or CUTL1 antibody) that detects the CDP protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. CDP Antibody (B-10) is available as both the non-conjugated anti-CDP antibody form, as well as multiple conjugated forms of anti-CDP antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. CDP (for CCAAT displacement protein) has been identified as a repressor for transcription of developmentally regulated genes. It is a homeodomain protein that appears to compete with transcriptional activating proteins for binding to the promoter regions of various genes. CDP contains three cut repeats which function as DNA binding domains. It has been demonstrated that cut repeat domains have the capacity to bind to DNA in conjunction with or independently of homeodomain DNA binding. CDP has been shown to be the DNA-binding subunit of the HiNF-D complex, which contains cyclin A, Cdc2 and an Rb-related protein, in addition to CDP. Histone expression is required for the transition to S phase in the cell cycle. The HiNF-D complex regulates the transcription of histone H4, H3 and H1 genes, allowing cells to progress from G1 to S phase.

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References:

1. Human CCAAT displacement protein is homologous to the Drosophila homeoprotein, cut.  |  Neufeld, EJ., et al. 1992. Nat Genet. 1: 50-5. PMID: 1301999
2. Antagonistic regulation of the Drosophila PCNA gene promoter by DREF and Cut.  |  Seto, H., et al. 2006. Genes Cells. 11: 499-512. PMID: 16629902
3. Differentiation-induced cleavage of Cutl1/CDP generates a novel dominant-negative isoform that regulates mammary gene expression.  |  Maitra, U., et al. 2006. Mol Cell Biol. 26: 7466-78. PMID: 17015474
4. Transgenic mice expressing the p75 CCAAT-displacement protein/Cut homeobox isoform develop a myeloproliferative disease-like myeloid leukemia.  |  Cadieux, C., et al. 2006. Cancer Res. 66: 9492-501. PMID: 17018605
5. Conserved cut repeats in the human cut homeodomain protein function as DNA binding domains.  |  Harada, R., et al. 1994. J Biol Chem. 269: 2062-7. PMID: 7904999
6. The mouse homeodomain protein Phox2 regulates Ncam promoter activity in concert with Cux/CDP and is a putative determinant of neurotransmitter phenotype.  |  Valarchε, I., et al. 1993. Development. 119: 881-96. PMID: 7910552
7. CCAAT displacement protein competes with multiple transcriptional activators for binding to four sites in the proximal gp91phox promoter.  |  Luo, W. and Skalnik, DG. 1996. J Biol Chem. 271: 18203-10. PMID: 8663528
8. CDP/cut is the DNA-binding subunit of histone gene transcription factor HiNF-D: a mechanism for gene regulation at the G1/S phase cell cycle transition point independent of transcription factor E2F.  |  van Wijnen, AJ., et al. 1996. Proc Natl Acad Sci U S A. 93: 11516-21. PMID: 8876167

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CUTL1/CUX1/CDP Antibody (B-10) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-CUTL1/CUX1/CDP Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CUTL1/CUX1/CDP Antibody (B-10) Specifications

Alternate Product Name

CDP

Product Clone Name

B-10

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₃ kappa light chain

Amino Acids

1308-1332

Epitope Target

CDP

Epitope Species

mouse

Product Reactivity/Detection

CDP

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

18 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG₃ Binding Proteins

• m-IgG₃ BP-HRP: sc-533670 (Mouse IgG₃ binding protein conjugated to horseradish peroxidase)
• m-IgG₃ BP-B: sc-533672 (Mouse IgG₃ binding protein conjugated to biotin)
• m-IgG₃ BP-FITC: sc-533671 (Mouse IgG₃ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₃ BP-CFL 488: sc-533673 (Mouse IgG₃ binding protein conjugated to CruzFluor™ 488)
• m-IgG₃ BP-CFL 555: sc-533674 (Mouse IgG₃ binding protein conjugated to CruzFluor™ 555)
• m-IgG₃ BP-CFL 594: sc-533675 (Mouse IgG₃ binding protein conjugated to CruzFluor™ 594)
• m-IgG₃ BP-CFL 647: sc-533676 (Mouse IgG₃ binding protein conjugated to CruzFluor™ 647)
• m-IgG₃ BP-CFL 680: sc-533677 (Mouse IgG₃ binding protein conjugated to CruzFluor™ 680)
• m-IgG₃ BP-CFL 790: sc-533678 (Mouse IgG₃ binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent