ACAT-1 Antibody (AT15E5): sc-517387

ACAT-1 Antibody (AT15E5) is an IgG₁ κ mouse monoclonal ACAT-1 antibody (also designated ACAT-1 antibody) that detects the ACAT-1 protein of human origin by WB, IP, IF and ELISA. ACAT-1 Antibody (AT15E5) is available as the non-conjugated anti-ACAT-1 antibody. ACAT-1 (acetyl-Coenzyme A acetyltransferase 1) is a mitochondrial enzyme involved in the formation and degradation of ketone bodies and is necessary for the proper metabolic processing of isoleucine. Rare defects in the gene encoding ACAT-1 lead to β-ketothiolase deficiency, which is characterized by ketoacidotic attacks. ACAT-2 (acetyl-Coenzyme A acetyltransferase 2) is considered a cytosolic protein and is crucial for cholesterol synthesis. Specifically, both Acetoacetyl-CoA specific thiolases, ACAT-1 and ACAT-2 catalyze the formation of acetoacetyl-CoA from two acetyl-CoA molecules. These enzymes are also capable of the reverse reaction, the cleavage of acetoacetyl-CoA into two acetyl-CoA molecules.

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References:

1. Identification, purification and characterization of an acetoacetyl-CoA thiolase from rat liver peroxisomes.  |  Antonenkov, VD., et al. 2000. Eur J Biochem. 267: 2981-90. PMID: 10806397
2. Phylogenetic analysis of the thiolase family. Implications for the evolutionary origin of peroxisomes.  |  Igual, JC., et al. 1992. J Mol Evol. 35: 147-55. PMID: 1354266
3. High resolution crystal structures of human cytosolic thiolase (CT): a comparison of the active sites of human CT, bacterial thiolase, and bacterial KAS I.  |  Kursula, P., et al. 2005. J Mol Biol. 347: 189-201. PMID: 15733928
4. Phylogenetic analysis of eukaryotic thiolases suggests multiple proteobacterial origins.  |  Peretó, J., et al. 2005. J Mol Evol. 61: 65-74. PMID: 15980957
5. Inborn errors of isoleucine degradation: a review.  |  Korman, SH. 2006. Mol Genet Metab. 89: 289-99. PMID: 16950638
6. Crystallographic and kinetic studies of human mitochondrial acetoacetyl-CoA thiolase: the importance of potassium and chloride ions for its structure and function.  |  Haapalainen, AM., et al. 2007. Biochemistry. 46: 4305-21. PMID: 17371050
7. Rat liver peroxisomes catalyze the initial step in cholesterol synthesis. The condensation of acetyl-CoA units into acetoacetyl-CoA.  |  Thompson, SL. and Krisans, SK. 1990. J Biol Chem. 265: 5731-5. PMID: 1969406
8. Assignment of the human cytosolic acetoacetyl-coenzyme A thiolase (ACAT2) gene to chromosome 6q25.3-q26.  |  Masuno, M., et al. 1996. Genomics. 36: 217-8. PMID: 8812443

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ACAT-1 Antibody (AT15E5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-ACAT-1 Antibody (AT15E5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ACAT-1 Antibody (AT15E5) Specifications

Alternate Product Name

ACAT-1

Product Clone Name

AT15E5

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

34-427

Epitope Target

ACAT-1

Epitope Species

human

Product Reactivity/Detection

ACAT-1

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, ELISA

Vialing Information

Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide, 0.1% gelatin and 1% glycerol

Research Citations

1 citations in various scientific publications

Recommended Secondary Detection Reagents

N/A

Available Conjugates/Forms

N/A