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Evaluation of 18F-AlF-labeled IF7 dimer as a promising molecular probe for tumor targeting PET imaging in mice

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Abstract

Molecular imaging technologies, such as PET imaging, have evolved into powerful tools for tumor diagnosis. Radiolabeled dimeric molecular probes have emerged as a promising strategy in PET imaging due to their outstanding characteristics. IF7, a peptide targeting Anxa1 overexpressed in endothelial cells of various tumor blood vessels, serves as an excellent tumor-targeting agent. This research aims to prepare IF7 dimer and evaluate its imaging performance and biological characteristics. The biological properties of the tracer were evaluated through in vitro experiments using U87 cells. MicroPET imaging and biodistribution were also studied in tumor-bearing mice. MicroPET studies showed a high tumor uptake at 30 and 60 min post-injection with 4.09 ± 0.83%ID/g and 2.66 ± 0.55%ID/g, respectively. Co-injection of excess nonradiolabelled peptide significantly reduced tumor uptake at 30 min (0.33 ± 0.15%ID/g), confirming the tumor-targeting specificity of IF7 dimer. The IF7 dimer tracer demonstrated excellent imaging performance as a tumor-targeting molecular probe, indicating potential clinical applicability and offering new possibilities for future early tumor diagnosis.

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Acknowledgements

The authors are grateful to the Jiangsu Institute of Nuclear Medicine (NHC Key Laboratory of Nuclear Medicine).

Funding

We are grateful that this work is supported by the Wuxi Municipal Health Commission General Project (M202230) and the Wuxi Medical Centre Project (WMCG202333).

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  1. Zhigang Du and Xinyu Xue equally contribute to the work.

Authors and Affiliations

  1. Department of Nuclear Medicine, the Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi People’s Hospital, Wuxi Medical Center, Nanjing Medical University, Wuxi, 214023, Jiangsu, People’s Republic of China

    Zhigang Du, Xinyu Xue, Wenfang Liao, Qinyue Gao, Fei Chen & Bao Zhu

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  1. Zhigang Du
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Du, Z., Xue, X., Liao, W. et al. Evaluation of 18F-AlF-labeled IF7 dimer as a promising molecular probe for tumor targeting PET imaging in mice. J Radioanal Nucl Chem 333, 2059–2068 (2024). https://doi.org/10.1007/s10967-024-09391-z

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