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Yaniella soli sp. nov., a new actinobacterium isolated from non-saline forest soil in China

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Abstract

A novel Gram-stain-positive, slightly halophilic, facultatively alkaliphilic, non-motile, non-sporulating, catalase-positive, oxidase-negative, aerobic bacterium, designated strain JSM 070026T, was isolated from non-saline forest soil in China. Growth occurred with 0–20% (w/v) NaCl (optimum, 2–4%) and at pH 6.0–10.5 (optimum, pH 8.0) and 5–40°C (optimum, 30°C). Good growth also occurred in the presence of 0–28% (w/v) KCl (optimum, 2–5%) or 0–25% (w/v) MgCl2·6H2O (optimum, 1–4%). The peptidoglycan type was A4α (l-Lys–Gly–l-Glu). Cell-wall sugars contained mannose and xylose. The major cellular fatty acids were anteiso-C15:0 and iso-C15:0. Strain JSM 070026T contained menaquinone 8 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major polar lipids. The DNA G + C content of strain JSM 070026T was 56.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JSM 070026T was a member of the suborder Micrococcineae and most closely related to Yaniella flava YIM 70178T (sequence similarity 99.4%) and Yaniella halotolerans YIM 70085T (97.9%). The three strains formed a distinct branch in the phylogenetic tree. The combination of phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the proposal that strain JSM 070026T represents a novel species of the genus Yaniella, for which the name Yaniella soli sp. nov. is proposed. The type strain is JSM 070026T (=DSM 22211T = KCTC 13527T).

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Acknowledgements

This work was supported by grants from the National Basic Research Program of China (2010CB833800), National Natural Science Foundation of China (NSFC) (30970007), Jishou University (09JDY022) and International Cooperation Research Program of Yunnan Province (2009AC017). We are grateful to Mr Ke Huang for his excellent technical assistance.

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Authors and Affiliations

  1. College of Biology and Environmental Sciences, Department of Financial Management, Jishou University, 416000, Jishou, People’s Republic of China

    Yi-Guang Chen, Jun Chen, Qi-Hui Chen, Jian-Wu He & Yan-Qi Liu

  2. The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, People’s Republic of China

    Shu-Kun Tang & Wen-Jun Li

  3. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, 100050, Beijing, People’s Republic of China

    Yu-Qin Zhang

  4. Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 510301, Guangzhou, People’s Republic of China

    Wen-Jun Li

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  1. Yi-Guang Chen
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  2. Jun Chen
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  3. Qi-Hui Chen
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Corresponding author

Correspondence to Yan-Qi Liu.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 070026T is FJ527420.

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Chen, YG., Chen, J., Chen, QH. et al. Yaniella soli sp. nov., a new actinobacterium isolated from non-saline forest soil in China. Antonie van Leeuwenhoek 98, 395–401 (2010). https://doi.org/10.1007/s10482-010-9453-1

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