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AtARRE, an E3 ubiquitin ligase, negatively regulates ABA signaling in Arabidopsis thaliana

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Abstract

Key message

The RING-type E3 ligase AtARRE participates in the plant ABA responding as a negative regulator.

Abstract

Ubiquitination protease system (UPS) is significant in post-transcriptional regulation. In UPS, E3 ligase recognizes the substrate protein and mediates the polyubiquitin chain onto the substrate. Here, we identified a new gene, named Arabidopsis thaliana ABA-related RING-type E3 ligase (AtARRE), which induced by ABA and NaCl. AtARRE encodes a functional RING-type E3 ligase protein localized in nucleus and plasma membrane of Arabidopsis. Physiological analysis demonstrated that mutation of AtARRE (T-DNA insert mutants atarre-1 and atarre-2) caused plants hypersensitivity to ABA, including enhanced stomatal closure, reduced root elongation and seed germination. However, overexpression of AtARRE transgenic lines caused plants hyposensitive to ABA compared with WT and mutant atarre plants. Under the treatment of ABA, the transcript abundances of ABA-responsive genes RD29A, RD29B, RD22 and ABI5 in atarre mutant plants were markedly higher than those of WT and AtARRE overexpression lines. Hence, these results indicate that AtARRE acts as a negative regulator of ABA-mediated stress responses in Arabidopsis.

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Acknowledgements

This study was funded by NSFC31671455 (Y. Y.), 973 Projects 2015CB755702 (Y. Y.), national transgenic project 2016ZX08009003-002-001 (X. L.).

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Authors and Affiliations

  1. Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, China

    Boya Wang, Chuzhe Li, Xiangge Kong, Ying Li, Zhibin Liu, Jianmei Wang, Xufeng Li & Yi Yang

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  1. Boya Wang
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  2. Chuzhe Li
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  3. Xiangge Kong
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  4. Ying Li
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  5. Zhibin Liu
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  8. Yi Yang
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Correspondence to Yi Yang.

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Wang, B., Li, C., Kong, X. et al. AtARRE, an E3 ubiquitin ligase, negatively regulates ABA signaling in Arabidopsis thaliana. Plant Cell Rep 37, 1269–1278 (2018). https://doi.org/10.1007/s00299-018-2311-8

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  • DOI: https://doi.org/10.1007/s00299-018-2311-8

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