Abstract
Free full text
Proteins induced by aerobiosis in Escherichia coli.
Abstract
The role of protein induction and repression in the adaptation of Escherichia coli to changes in the supply of oxygen and other electron acceptors is only poorly understood. We have studied the changes in cellular protein composition associated with this adaptation by measuring the levels of 170 individual polypeptides produced during aerobic or anaerobic growth of E. coli, with and without nitrate. Nineteen polypeptides had levels highest during aerobic growth. These proteins include the enzymes of the pyruvate dehydrogenase complex, several tricarboxylic acid cycle enzymes, superoxide dismutase, and tetrahydropteroyltriglutamate transmethylase. The other aerobiosis-induced proteins have not been identified. These polypeptides are major cellular proteins during aerobic growth and display several different patterns of regulation in response to medium composition. Induction ratios for oxygen ranged from 2.2 to 11.2, with one exceptional member, superoxide dismutase, increasing 71-fold with aeration. Most of the proteins were also induced by nitrate during anaerobic growth. The time course of induction after shifts in oxygen supply revealed similarities in response among proteins of related function or metabolic regulation class. These results are discussed in relation to previously reported information on the identified aerobiosis-induced proteins.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Images in this article
Click on the image to see a larger version.
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bachmann BJ, Low KB. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. [Europe PMC free article] [Abstract] [Google Scholar]
- Bloch PL, Phillips TA, Neidhardt FC. Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins. J Bacteriol. 1980 Mar;141(3):1409–1420. [Europe PMC free article] [Abstract] [Google Scholar]
- Dietrich J, Henning U. Regulation of pyruvate dehydrogenase complex synthesis in Escherichia coli K 12. Identification of the inducing metabolite. Eur J Biochem. 1970 Jun;14(2):258–269. [Abstract] [Google Scholar]
- Dills SE, Dobrogosz WJ. Cyclic adenosine 3',5'-monophosphate regulation of membrane energetics in Escherichia coli. J Bacteriol. 1977 Sep;131(3):854–865. [Europe PMC free article] [Abstract] [Google Scholar]
- Gray CT, Wimpenny JW, Hughes DE, Mossman MR. Regulation of metabolism in facultative bacteria. I. Structural and functional changes in Escherichia coli associated with shifts between the aerobic and anaerobic states. Biochim Biophys Acta. 1966 Mar 28;117(1):22–32. [Abstract] [Google Scholar]
- Guest JR, Stephens PE. Molecular cloning of the pyruvate dehydrogenase complex genes of Escherichia coli. J Gen Microbiol. 1980 Dec;121(2):277–292. [Abstract] [Google Scholar]
- Haddock BA, Jones CW. Bacterial respiration. Bacteriol Rev. 1977 Mar;41(1):47–99. [Europe PMC free article] [Abstract] [Google Scholar]
- Hassan HM, Fridovich I. Enzymatic defenses against the toxicity of oxygen and of streptonigrin in Escherichia coli. J Bacteriol. 1977 Mar;129(3):1574–1583. [Europe PMC free article] [Abstract] [Google Scholar]
- Hassan HM, Fridovich I. Regulation of the synthesis of superoxide dismutase in Escherichia coli. Induction by methyl viologen. J Biol Chem. 1977 Nov 10;252(21):7667–7672. [Abstract] [Google Scholar]
- Hespell RB. Glycolytic and tricarboxylic acid cycle enzyme activities during intraperiplasmic growth of Bdellovibrio bacteriovorus on Escherichia coli. J Bacteriol. 1976 Nov;128(2):677–680. [Europe PMC free article] [Abstract] [Google Scholar]
- Krebs HA. The Pasteur effect and the relations between respiration and fermentation. Essays Biochem. 1972;8:1–34. [Abstract] [Google Scholar]
- Langley D, Guest JR. Biochemical genetics of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: isolation and biochemical properties of deletion mutants. J Gen Microbiol. 1977 Apr;99(2):263–276. [Abstract] [Google Scholar]
- MAAS WK. STUDIES ON THE MECHANISM OF REPRESSION OF ARGININE BIOSYNTHESIS IN ESCHERICHIA COLI. II. DOMINANCE OF REPRESSIBILITY IN DIPLOIDS. J Mol Biol. 1964 Mar;8:365–370. [Abstract] [Google Scholar]
- Mulligan JT, Margolin W, Krueger JH, Walker GC. Mutations affecting regulation of methionine biosynthetic genes isolated by use of met-lac fusions. J Bacteriol. 1982 Aug;151(2):609–619. [Europe PMC free article] [Abstract] [Google Scholar]
- Pedersen S, Bloch PL, Reeh S, Neidhardt FC. Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates. Cell. 1978 May;14(1):179–190. [Abstract] [Google Scholar]
- Sanwal BD. Allosteric controls of amphilbolic pathways in bacteria. Bacteriol Rev. 1970 Mar;34(1):20–39. [Europe PMC free article] [Abstract] [Google Scholar]
- Smith MW, Neidhardt FC. Proteins induced by anaerobiosis in Escherichia coli. J Bacteriol. 1983 Apr;154(1):336–343. [Europe PMC free article] [Abstract] [Google Scholar]
- Takahashi Y. Effect of glucose and cyclic adenosine 3',5'-monophosphate on the synthesis of succinate dehydrogenase and isocitrate lyase in Escherichia coli. J Biochem. 1975 Nov;78(5):1097–1100. [Abstract] [Google Scholar]
- Thomas AD, Doelle HW, Westwood AW, Gordon GL. Effect of oxygen on several enzymes involved in the aerobic and anaerobic utilization of glucose in Escherichia coli. J Bacteriol. 1972 Dec;112(3):1099–1105. [Europe PMC free article] [Abstract] [Google Scholar]
- Wimpenny JW, Cole JA. The regulation of metabolism in facultative bacteria. 3. The effect of nitrate. Biochim Biophys Acta. 1967 Oct 9;148(1):233–242. [Abstract] [Google Scholar]
- Yamamoto I, Ishimoto M. Effect of nitrate reduction on the enzyme levels in carbon metabolism in Escherichia coli. J Biochem. 1975 Aug;78(2):307–315. [Abstract] [Google Scholar]
Associated Data
Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)
Full text links
Read article at publisher's site: https://doi.org/10.1128/jb.154.1.344-350.1983
Read article for free, from open access legal sources, via Unpaywall: https://jb.asm.org/content/jb/154/1/344.full.pdf
Free to read at jb.asm.org
http://jb.asm.org/cgi/content/abstract/154/1/344
Free after 4 months at jb.asm.org
http://jb.asm.org/cgi/reprint/154/1/344
Citations & impact
Impact metrics
Citations of article over time
Smart citations by scite.ai
Explore citation contexts and check if this article has been
supported or disputed.
https://scite.ai/reports/10.1128/jb.154.1.344-350.1983
Article citations
Fitness trade-offs of multidrug efflux pumps in Escherichia coli K-12 in acid or base, and with aromatic phytochemicals.
Appl Environ Microbiol, 90(2):e0209623, 30 Jan 2024
Cited by: 0 articles | PMID: 38289137 | PMCID: PMC10880634
Pan-Genome Analysis of Transcriptional Regulation in Six Salmonella enterica Serovar Typhimurium Strains Reveals Their Different Regulatory Structures.
mSystems, 7(6):e0046722, 01 Nov 2022
Cited by: 3 articles | PMID: 36317888 | PMCID: PMC9764980
Machine learning from Pseudomonas aeruginosa transcriptomes identifies independently modulated sets of genes associated with known transcriptional regulators.
Nucleic Acids Res, 50(7):3658-3672, 01 Apr 2022
Cited by: 17 articles | PMID: 35357493 | PMCID: PMC9023270
Machine Learning of All Mycobacterium tuberculosis H37Rv RNA-seq Data Reveals a Structured Interplay between Metabolism, Stress Response, and Infection.
mSphere, 7(2):e0003322, 21 Mar 2022
Cited by: 16 articles | PMID: 35306876 | PMCID: PMC9044949
Inverted Regulation of Multidrug Efflux Pumps, Acid Resistance, and Porins in Benzoate-Evolved Escherichia coli K-12.
Appl Environ Microbiol, 85(16):e00966-19, 01 Aug 2019
Cited by: 9 articles | PMID: 31175192 | PMCID: PMC6677852
Go to all (74) article citations
Similar Articles
To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.
Proteins induced by anaerobiosis in Escherichia coli.
J Bacteriol, 154(1):336-343, 01 Apr 1983
Cited by: 115 articles | PMID: 6339476 | PMCID: PMC217464
Cellular and molecular physiology of Escherichia coli in the adaptation to aerobic environments.
J Biochem, 120(6):1055-1063, 01 Dec 1996
Cited by: 60 articles | PMID: 9010748
Review
Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors.
Biochim Biophys Acta, 1320(3):217-234, 01 Jul 1997
Cited by: 359 articles | PMID: 9230919
Review
Nitric oxide, nitrite, and Fnr regulation of hmp (flavohemoglobin) gene expression in Escherichia coli K-12.
J Bacteriol, 178(18):5487-5492, 01 Sep 1996
Cited by: 129 articles | PMID: 8808940 | PMCID: PMC178372